Purification and Characterization of Fibrinolytic Enzyme from Lepista nuda
نویسندگان
چکیده
منابع مشابه
Purification and Characterization of a White Laccase with Pronounced Dye Decolorizing Ability and HIV-1 Reverse Transcriptase Inhibitory Activity from Lepista nuda.
A strain LN07 with high laccase yield was identified as basidiomycete fungus Lepista nuda from which a white laccase without type I copper was purified and characterized. The laccase was a monomeric protein with a molecular mass of 56 kDa. Its N-terminal amino acid sequence was AIGPAADLHIVNKDISPDGF. Besides, eight inner peptide sequences were determined and lac4, lac5 and lac6 sequences were in...
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Blood clots are formed from fibrinogen by thrombin and are lysed by plasmin, which is activated from tissue plasminogen activator. It was developed for the treatment of thrombosis because of its efficacy and strong affinity to fibrin. Due to the expense and undesirable side effects such as gastrointestinal bleeding, allergic reaction, and resistance to repercussion. Therefore the search for thr...
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Intravascular thrombosis is one of the major causes of variety of cardiovascular disorders leading to high mortality worldwide. Fibrinolytic enzymes from microbial sources possess ability to dissolve these clots and help to circumvent these problems in more efficient and safer way. In the present study, fibrinolytic protease with higher fibrinolytic activity than plasmin was obtained from Serra...
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BACKGROUND & OBJECTIVE Treatment of thromboembolic vascular disease has relied on anticoagulants. However, recognition that lysis of preformed fibrin could be accomplished in vivo by a process involving the conversion of inactive plasminogen to active plasmin enzyme led to an alternative enzyme-based approach. The drugs used for this therapy are called the fibrinolytic enzymes. In this study we...
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Bacillus sp. strain CK 11-4, which produces a strongly fibrinolytic enzyme, was screened from Chungkook-Jang, a traditional Korean fermented-soybean sauce. The fibrinolytic enzyme (CK) was purified from supernatant of Bacillus sp. strain CK 11-4 culture broth and showed thermophilic, hydrophilic, and strong fibrinolytic activity. The optimum temperature and pH were 70 degrees C and 10.5, respec...
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ژورنال
عنوان ژورنال: The Korean Journal of Mycology
سال: 2005
ISSN: 0253-651X
DOI: 10.4489/kjm.2005.33.2.069